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1: Appl Biochem Biotechnol. 2006 Nov;135(2):133-44.Compound
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Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein.
Department of Genetic Engineering, Soonchunhyang
University, Asan, Chungnam 336-600, Korea. miyoung@sch.ac.kr
The generation of reactive oxygen
species is thought to cause extensive oxidative damage to various biomolecules
such as DNA, RNA, and protein. In this study, the preventive, suppressive, and
protective effects of in vitro supplementation with electrolyzed-reduced water
on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay.
Pretreatment, cotreatment, and posttreatment with electrolyzed-reduced water
enhanced human lymphocyte resistance to the DNA strand
breaks induced by H2O2 in vitro.
Moreover, electrolyzed-reduced water was
much more effective than diethylpyrocarbonate-treated water in preventing total
RNA degradation at 4 and 25 degrees C. In
addition, electrolyzed-reduced water completely prevented the oxidative
cleavage of horseradish peroxidase, as determined using sodium dodecyl
sulfate-polyacrylamide gels. Enhancement of the antioxidant activity
of ascorbic acid dissolved in electrolyzed-reduced water was about threefold that
of ascorbic acid dissolved in nonelectrolyzed deionized
water, as measured by a xanthine-xanthine oxidase superoxide
scavenging assay system, suggesting an inhibitory effect of electrolyzedreduced
water on the oxidation of ascorbic acid.
PMID: 17159237 [PubMed - indexed
for MEDLINE]
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Electrolyzed-reduced
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[Biochem Biophys Res Commun. 1997]
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