Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the l-methyl-4-phenyl-l,2,3,6tetrahydropyridine Mouse Model of Parkinson's Disease

Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the l-methyl-4-phenyl-l,2,3,6tetrahydropyridine Mouse Model of Parkinson's Disease 

KyotaFujita, Toshihiro Seike, Noriko Yutsudo,Mizuki Ohno,Hidetaka Yamada, Hiroo Yamaguchi, Kunihiko Sakumi, Yukiko Yamakawa, Mizuho A. Kido, Atsushi Takakl, Toshihiko Katafuchl, Yoshinori Tanakas, Yusaku Nakabeppu, Mami Noda

Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan,Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan,Department of Oral Anatomy and Cell Biology, Graduate School of Dental Sciences,Kyushu University, Fukuoka, Japan,Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan,R&D Center, Home Appliances Manufacturing BusinessUnit, PanasonicElectricWorksCo.,Ltd.,Osaka,Japan

Abstract

 It has been shown that molecular hydrogen (H2) acts as a therapeutic antioxidant and suppresses brain injury by buffering the effects of oxidative stress. Chronic oxidative stress causes neurodegenerative diseases such as Parkinson's disease (PD). Here, we show that drinking H2-containing water significantly reduced the loss of dopaminergic neurons in PD model mice using both acute and chronic administration of l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP). 

The concentration dependency of H2 showed that H2 as low as 0.08 ppm had almost the same effect as saturated H2 water (1.5 ppm). MPTP induced accumulation of cellular 8-oxoguanine (8-oxoG), a marker of DNA damage, and 4-hydroxynonenal (4-HNE), a marker of lipid peroxidation were significantly decreased in the nigro-striatal dopaminergic pathway in mice drinking Hr containing water, whereas production of superoxide (O2•-) detected by intravascular injection of dihydroethidium (DHE) was not reduced significantly.

 Our results indicated that low concentration of H2 in drinking water can reduce oxidative stress in the brain. Thus, drinking H2-containing water may be useful in daily life to prevent or minimize the risk of life stylerelated oxidative stress and neuro-degeneration.

See Hydrogen In Drinking Water.pdf for the paper.